Immunofluorescent Identification of Mycoplasma Colonies Grown on Agar-Covered Glass Slides

Morphology of Leishmania donovani colonies grown on blood agar plates.

In situ cytoenzymatic identification of human bone marrow colonies grown in agar: a simple method with automated cytochemistry reagents.

Human bone marrow culture in agar is a widely used technique for the study of haemopoietic progenitor cells in both normal and pathological states.' The colonies formed are heterogeneous, consisting of neutrophil, monocyte, eosinophil, and mixed neutrophil monocyte colonies. Methods for differentiating colony types have been described,1'8 but they are cumbersome, require removal of the agar fro...

Glass slides to DNA microarrays

on genomics, became aware of the potential commercial, technical, and social importance of the human genome project during the late 1990s. The human genome project was formally initiated in 19901 and was expected to last 15 years. It had the major goals of identifying all the genes in human DNA, determining the sequences of those genes, and storing the information in public databases. However, ...

Immunofluorescent identification of human megakaryocyte colonies using an antiplatelet glycoprotein antiserum.

The development of a satisfactory in vitro assay system for human megakaryocyte colony forming progenitor cells has been delayed by the lack of a suitable marker for cells of human megakaryocyte lineage. For this purpose we raised an antiserum directed against a purified human platelet glycoprotein preparation. In conjunction with indirect immunofluorescent staining of human bone marrow, this a...

An Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays

Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...